i.Mune NEO is a quantitative in vitro diagnostic test to determine the percentages of lymphocyte subsets in newborn capillary whole blood specimens collected and dried on filter paper.
The test uses a real-time polymerase chain reaction (PCR) for the detection of immune cell type specific demethylated genomic target regions associated with the following immune cell types::
The i.Mune NEO [CE] test consists of the following kits:
Epigenetic quantification of CD3+ T lymphocytes for second-tier testing after conspicuous SCID (Severe Combined Immunodeficiency) screening findings (Blom et al., 2021)1)
Second tier testing using epigenetic immune cell quantification can reduce the number of non-actionable secondary findings and false-positive referrals in newborn screening for SCID.